Agarose Strep-tag Ⅱ Protein Purification Magbeads 30-150 μm, 10% (v/v)
1, Description:
The Strep Tactin beads based on superparamagnetic beads are designed for rapid and efficient separation Strep(II)-tag protein. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). Generally, it does not interfere with folding or bioactivity. Thus, there is no need for removing the tag. Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. The proteins obtained are bioactive and display a very high purity (above 99%).
2, Product Information:
| Product Name |
Agarose Strep Tactin Magbeads
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Bead Size
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30~150 μm
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Ligand density
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~6 mg Strep-Tactin / mL Gel
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| Protein binding Capacity (mg/ml pure beads) |
~7 mg Strep-tag Ⅱprotein / mL Gel
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| Suspension concentration |
10%(v/v)magnetic beads suspension
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Binding Buffer
|
50 mM Tris-HCl, pH 8.0
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Elution Buffer
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2.5 mM desthiobiotin in Binding Buffer
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Storage buffer
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1×PBS (0.1% Tween-20+0.05% NaN3)
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Shelf life
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2 years when stores at 2 ~ 8 ℃,
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Regeneration Buffer
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0.5 M NaOH or 1 mM HABA in Binding Buffer
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3, Features:
1. High capability of antibody binding.
2. Time-saving, simple and mild.
3. Obtained high-purity protein by one-Step.
4. No need removing the tag.
