Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10% (v/v)
1, Description:
This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. The one-step solution can reduce the antibody absorption process to 10 minutes and purification process to 30 minutes, yielding antibody with >90% purity. It can be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.
2, Product Information:
| Product Name |
Magrose Protein A
|
|
Bead Size
|
30~150 μm
|
|
Beads Concentration
|
10% (v/v)
|
|
Ligand
|
Protein A
|
|
Medium
|
Magrose
|
|
Antibody Bonding Quantity
|
25~30 mg Human IgG/mL Gel
|
|
Storage Temperature
|
2~8℃
|
| Binding/Washing Buffer |
PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM
Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20
|
|
Elution buffer
|
100 mM Gly, 0.1% Tween-20, pH 2.5
|
|
Neutrilization buffer
|
1.0 M Tris-HCl, pH 9.0
|
|
Storage buffer
|
PBST (includes 0.05% NaN3)
|
|
Shelf life
|
12 months
|
3, Feature:
1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
