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Agrose Protein A Magnetic Beads For Protein Purification 10% Volume Ratio 500 mL

5 mL / bottle
Agrose Protein A Magnetic Beads For Protein Purification 10% Volume Ratio 500 mL
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Product Name: Magrose Protein A Magbeads
Application: Protein Purification
Shelf Life: 2 Years
Usage: Medical Research
Storage: 2~8℃ Sealed Storage
Package: 5mL/100mL/500mL
Concentration: 10% (v/v)
Specification: 500 ML, 10% (v/v), 30-150 μm
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Agrose Protein A Magnetic Beads


BeaverBeads 500 mL magnetic agarose beads


Protein A Magnetic Beads Protein Purification

Basic Infomation
Place of Origin: China
Brand Name: BeaverBeads
Certification: COA
Model Number: 70804-500
Payment & Shipping Terms
Packaging Details: 5mL/100mL/500mL
Delivery Time: 5-8 days
Payment Terms: L/C, T/T
Supply Ability: 850 L/month
Product Description

Magrose Protein A Magbeads for Protein Purification 30-150 μm, 10% (v/v)


1, Description:

This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. The one-step solution can reduce the antibody absorption process to 10 minutes and purification process to 30 minutes, yielding antibody with >90% purity. This product can be used repeatedly, which is suitable for the purification of antibodies in plasma, ascites, tissue culture supernatant and other samples.


2, Product Information:


Product Name
Magrose Protein A
Bead Size
30~150 μm
Beads Concentration
10% (v/v)
Protein A
Antibody Bonding Quantity
25~30 mg Human IgG/mL Gel
Storage Temperature
Binding/Washing Buffer
PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM
Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20
Elution buffer
100 mM Gly, 0.1% Tween-20, pH 2.5
Neutrilization buffer
1.0 M Tris-HCl, pH 9.0
Storage buffer
PBST (includes 0.05% NaN3)
Shelf life
12 months


3, Feature:

1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance

2. Time saving, simple and mild

3. High product stability

4. Antibody elution system is closer to neutral

5. Very low protein A ligand off rate

6. Reusable


4, FAQ:


Q1: How to improve the efficiency of antibody elution?
A1: When the high affinity between antibody and Protein A ligand result in antibody low elution efficiency, users can avoid this situation by reducing the pH value (1.9~2.5) of elution buffer, increasing the ionic strength of elution buffer (2~3 M MgCl2) or prolonging the elution time. But pay attention that antibody could easily aggregate under low pH conditions. Users can adjust the pH to neutral immediately with alkaline buffers (such as Tris, HEPES, etc.).
Q2: How to avoid possible aggregation of magnetic beads during storage or use?
A2: Beads should be stored at 2~8℃, and avoid for irreversible aggregation due to contamination or drying. Magnetic beads aggregation at low pH elution buffer is a normal phenomenon, and it does not affect the normal use of magnetic beads. In Binding/Washing buffer and Elution buffer adding with the final concentration of 0.1% (v/v) nonionic detergent (such as NP-40, Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to neutral with Binding/Washing buffer and Elution buffer, and process with 2 min ultrasonic water bath. It can restore the magnetic beads to homogeneity, and the above treatments had no effect on the antibody binding efficiency with magnetic beads.
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