Agarose Strep-tag Ⅱ Protein Purification Magbeads 30-150 μm, 10% (v/v)
1, Description:
Strep-Tag system is a new type of protein purification system which simulates the streptavidin-biotin system. Compared to streptavidin, Strep-Tactin's affinity to Strep-Tag II is at least 10 times larger. The purification extraction condition of strep-tag is mild and the protein can be separated and purified under physiological conditions. In addition, compared to other tags, Strep-Tag II is small 8 amino acid tag (WSHPQFEK). Since the tag is small, it is only about 1 kDa which doesn't affect the structure and function of the protein after fusion. These mild purification condition preserve the biological activity of the protein and produce more than 99% purity in only one extraction step.
2, Product Information:
| Product Name |
Agarose Strep Tactin Magbeads
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Bead Size
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30~150 μm
|
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Ligand density
|
~6 mg Strep-Tactin / mL Gel
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| Protein binding Capacity (mg/ml pure beads) |
~7 mg Strep-tag Ⅱprotein / mL Gel
|
| Suspension concentration |
10%(v/v)magnetic beads suspension
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|
Binding Buffer
|
50 mM Tris-HCl, pH 8.0
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Elution Buffer
|
2.5 mM desthiobiotin in Binding Buffer
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Storage buffer
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1×PBS (0.1% Tween-20+0.05% NaN3)
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Shelf life
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2 years when stores at 2 ~ 8 ℃,
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Regeneration Buffer
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0.5 M NaOH or 1 mM HABA in Binding Buffer
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3, Features:
1. High capability of antibody binding.
2. Time-saving, simple and mild.
3. Obtained high-purity protein by one-Step.
4. No need removing the tag.
