Agarose Strep-tag Ⅱ Protein Purification Magbeads 30-150 μm, 10% (v/v)
1, Description:
Strep-Tag system is a new type of protein purification system which simulates the streptavidin-biotin system. Compared to streptavidin, Strep-Tactin's affinity to Strep-Tag II is at least 10 times larger. The purification extraction condition of strep-tag is mild and the protein can be separated and purified under physiological conditions. In addition, compared to other tags, Strep-Tag II is small 8 amino acid tag (WSHPQFEK). Since the tag is small, it is only about 1 kDa which doesn't affect the structure and function of the protein after fusion. These mild purification condition preserve the biological activity of the protein and produce more than 99% purity in only one extraction step.
BeaverBeadsTM Magrose Strep-Tactin beads are using a special technology to couple the Strep-Tactin protein to the surface of the superparamagnetic magnetic beads. This new type of functionalized material has built an efficient protein purification platform that has possessed the advantage of extraction speed, extraction volume, and purity.
2, Product Information:
| Product Name |
Agarose Strep Tactin Magbeads
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Bead Size
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30~150 μm
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Ligand density
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~6 mg Strep-Tactin / mL Gel
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| Protein binding Capacity (mg/ml pure beads) |
~7 mg Strep-tag Ⅱprotein / mL Gel
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| Suspension concentration |
10%(v/v)magnetic beads suspension
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Binding Buffer
|
50 mM Tris-HCl, pH 8.0
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Elution Buffer
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2.5 mM desthiobiotin in Binding Buffer
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Storage buffer
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1×PBS (0.1% Tween-20+0.05% NaN3)
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Shelf life
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2 years when stores at 2 ~ 8 ℃,
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Regeneration Buffer
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0.5 M NaOH or 1 mM HABA in Binding Buffer
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3, Features:
1. High capability of antibody binding.
2. Time-saving, simple and mild.
3. Obtained high-purity protein by one-Step.
4. No need removing the tag.
