5 mL 100 mL 1000 mL BeaverBeads GSH with New Functional Material Designed
1. Description
BEAVER GST fusion protein purified beads are a new functional material designed for efficient and rapid purification of glutathione S-transferase (GST) fusion protein. It can extract the target protein with high purity from the biological samples in one step using magnetic separation method and it greatly simplifies the purification process and improves the efficiency of purification. This method is suitable for scientific research and industrial field to purify GST fusion protein.
Compared with the traditional column chromatography purification method, Beaverbeads method does not need to carry out high-speed long time centrifugation and filter filtration to the crude protein samples, or control the flow rate, and does not need the expensive chromatography equipment. The specific binding of the sample to the magnetic beads, washing and elution of target protein become very simple, fast and easy to operate. For skilled operators, high purified protein can be obtained within 1 h, and parallel processing of large scale samples can be easily achieved to save researchers time and cost.
2.Product Information
Product name | BeaverBeads™ GSH Cat.# 70601 |
BeaverBeads™ GSH kit Cat.# 70601-kit
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Component | BeaverBeads™ GSH | BeaverBeads™ GSH | 5 mL |
Buffer A | 100 mL | ||
Glutathione | 307 mg | ||
Buffer B | 100 mL | ||
Bead size | 30μm~150μm | ||
GSH ligand content | 20~30 μmol/mL(100% beads) | ||
GST fusion protein binding capacity | ≥5mg/mL (100% beads) | ||
Suspension concentration | 10%(V/V)magnetic beads suspension | ||
Preserving fluid | Pure Water (recommend to swtich to 20%V/V ethanol solution) | ||
Chemical stability |
At room temperature can tolerate 1 h of 70% ethanol, 6 M hydrochloride, 0.1 M hydroxide, 0.1 M acetic acid |
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Shelf Life | 2 years when stores at 2 ~ 8 ℃ |
*Note: 1.The amount of magnetic beads protein binding is related to the target protein characteristics, where only reference values are given.
2.1 mL magnetic beads suspension contain 100 μL of magnetic beads.
3. Application Case
4. Product List
Cat. No. | Product name | Specification |
70601-5 | BeaverBeadsTM GSH | 5 mL, 10% (v/v), 30-150 μm |
70601-K10 | 5 mL, 10% (v/v), 30-150 μm | |
70601-100 | 2×50 mL, 10% (v/v), 30-150 μm | |
70601-1000 | 4×250 mL, 10% (v/v), 30-150 μm |