Magrose Protein A Magbeads for Protein Purification 30-150 μm, 10% (v/v)
1, Description:
BeaverBeads™ Protein A, Protein G are superparamagnetic beads covalently coated with recombinant Protein A or Protein G that contains Fc binding domains for the purification of monoclonal or polyclonal antibodies from cell lysate, blood plasma, ascites and tissue culture supernatant, and for antibody immobilization studies. This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. It can be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.
2, Product Information:
| Product Name |
Magrose Protein A
|
|
Bead Size
|
30~150 μm
|
|
Beads Concentration
|
10% (v/v)
|
|
Ligand
|
Protein A
|
|
Medium
|
Magrose
|
|
Antibody Bonding Quantity
|
25~30 mg Human IgG/mL Gel
|
|
Storage Temperature
|
2~8℃
|
| Binding/Washing Buffer |
PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM
Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20
|
|
Elution buffer
|
100 mM Gly, 0.1% Tween-20, pH 2.5
|
|
Neutrilization buffer
|
1.0 M Tris-HCl, pH 9.0
|
|
Storage buffer
|
PBST (includes 0.05% NaN3)
|
|
Shelf life
|
12 months
|
3, Feature:
1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
