His-tag Protein Purification IDA-Nickel Magbeads 30-150 μm, 10% (v/v)
1, Description:
BeaverBeads™ IDA contains BeaverBeads™ IDA-Nickel and BeaverBeads™ IDA-Cobalt, Which are used for His-tagged protein affinity purification in either manual operation setting (with Magnetic Separators from BEAVER or other suppliers) or robotic automation setting (on magnetic separation workstations from Apricot Designs, Tecan or Hamilton, etc.).
2, Product Information:
| Product Name |
IDA-Nickel Magbeads
|
|
Bead Size
|
30~150 μm
|
|
Chelating metal ion
|
Ni2+
|
|
Metal ion density
|
30~50 µmol/mL beads
|
|
Protein binding ability
|
30~40 mg/mL (100% beads)
|
|
Operating temperature
|
2~30℃
|
|
Storage Temperature
|
2~8℃
|
|
Suspension concentration
|
10%(v/v)magnetic beads suspension
|
|
Storage buffer
|
Pure Water (recommend to swtich to 20%V/V ethanol)
|
|
Shelf life
|
12 months
|
3, Feature:
1. The target protein can be purified from the crude samples. Without high-speed centrifugation, filtration and the expensive chromatography equipment.
2. Using one-step can obtain high purity target protein.
3. The concentration and volume of protein can be easily controlled.
4. With high repeatability, high throughput and large-scale purification of proteins can be easiliy achieved.
5. Simple regeneration process.
6. High yield of target protein.
