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Agarose NHS Activated Magnetic Beads 20% Volume Ratio 5 mL

1 mL / bottle
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Agarose NHS Activated Magnetic Beads 20% Volume Ratio 5 mL
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Features
Specifications
Product Name: Agarose NHS Magbeads Preactivated
Bead Diameter: 30-150 μm
Concentration: 20% (v/v)
Ligand Content: ~50 μM/mL Gel
Binding Ability: 20~30 Mg Rabbit IgG/mL Beads
Magnetic Core: Fe3O4
Package: 1mL/5mL/50mL/1000mL
Specification: 5 ML, 20% (v/v), 30-150 μm
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Agarose NHS Activated Magnetic Beads

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150 um Agarose NHS Magbeads Preactivated

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5 mL NHS Activated Magnetic Beads

Basic Infomation
Place of Origin: China
Brand Name: BeaverBeads
Certification: COA
Model Number: 70702-5
Payment & Shipping Terms
Packaging Details: 1mL/5mL/50mL/1000mL
Delivery Time: 5-8 days
Payment Terms: T/T, L/C
Supply Ability: 850 L/month
Product Description

Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle​ 30 - 150 μm, 20% (v/v)

 

1, Description:

BeaverBeads ™ Mag NHS and BeaverBeads ™ Magrose NHS are spherical beads modified with NHS groups capable of forming stable peptide bonds with proteins and other molecules with primary amine groups for affinity purification or assay detection of antibodies, antigens and other biomolecules. Compared with the traditional beads with carboxyl or amino groups, the beads with surface containing NHS group do not need to be activated by EDC / NHS or glutaraldehyde in advance. Simply by dissolving the ligand containing the primary amino acid in the coupling buffer (mix the protein solution with Mag NHS beads at room temperature for 1 ~ 2h), the ligands will be attached on the beads.

 

2, Introduction: 

Product Name Agarose NHS Magbeads Preactivated
Mean particle size 30-150 μm
Magnetic core Fe3O4
Shell Agarose
Magnetic superparamagnetism
Ligand content ~50 μM/mL gel
Binding ability 20~30 mg rabbit IgG/mL beads
Preservation fluid 20% (v/v) ethanol solution
Operation Automate/manual
* water average particle size, Malvern Nano determination

 

3, FEATURES:

  • 1. Simple, without activation, can be directly covalently coupled with the biological ligand;

  • 2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;

  • 3. Fast, 1 ~ 2h to complete the bio-ligand coupling;

  • 4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;

  • 5. Stable, the formation of a stable amide bond, to prevent the ligand off.

  • 6. Good biocompatibility, reduced nonspecific adsorption.

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