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2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL

1 mL / bottle
2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL
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Product Name: Polymer NHS Magbeads Preactivated
Bead Diameter: 2 μm
Concentration: 10 Mg/mL
Magnetic Core: Fe3O4
Package: 1mL/5mL/50mL
Specification: 50 ML, 10 Mg/mL, 2 μm
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Polymer NHS Magbeads Preactivated


10 mg mL nhs magnetic beads


2 um Polymer NHS Magbeads Preactivated

Basic Infomation
Place of Origin: China
Brand Name: BeaverBeads
Certification: COA
Model Number: 70701-50
Payment & Shipping Terms
Packaging Details: 1mL/5mL
Delivery Time: 5-8 days
Payment Terms: T/T, L/C
Supply Ability: 850 L/month
Product Description

Polymer NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle​ 2 μm, 10 mg/mL


1, Description:

The NHS magnetic beads are superparamagnetic beads with activated groups of N_Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde, simply dissolving the amino-containing bio-ligand in the coupling buffer, at room temperature, mixing with proteins and the NHS magnetic beads for only 1-2 hours, the bio-ligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and coupling fast and efficient. The magnetic bead coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.


2, Introduction: 

Product Name Polymer NHS Magbeads Preactivated
Mean particle size 2 μm
Magnetic core Fe3O4
Shell Polymer
Magnetic superparamagnetism
Ligand content ~50 μM/mL gel
Binding ability 20~30 mg rabbit IgG/mL beads
Preservation fluid DMAC
Operation Automate/manual
* water average particle size, Malvern Nano determination



  • 1. Simple, without activation, can be directly covalently coupled with the biological ligand;

  • 2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;

  • 3. Fast, 1 ~ 2h to complete the bio-ligand coupling;

  • 4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;

  • 5. Stable, the formation of a stable amide bond, to prevent the ligand off.

  • 6. Good biocompatibility, reduced nonspecific adsorption.



4, Precautions:

1. Magnetic beads are sensitive to water. In order to ensure product quality, after sampling immediately cover
the bottle caps and seal with a sealing tape, store at 4 ℃.
2. Beads are not allowed to dry or freeze. Drying and freezing operations may result in the aggregation of
magnetic beads and thus loss of binding activity.
3. Pre-and post-reaction protein content can be determined by indirect methods (e.g., Thermo Scientific ™
Pierce ™ 660 nm Protein Assay, Product No. 22660 and 22662) or by direct methods (e.g., use the Thermo
Scientific ™ Pierce ™ Micro BCA Protein Assay, Product No. 23235) to detect the surface of the magnetic
beads coupled with the protein content. Using wavelengths around 280 nm to determine protein levels is
not advisable. Because NHS groups strongly absorb near 280nm, they can seriously interfere with the


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