Polymer NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle 2 μm, 10 mg/mL
BeaverBeads ™ Mag NHS and BeaverBeads ™ Magrose NHS are spherical beads modified with NHS groups capable of forming stable peptide bonds with proteins and other molecules with primary amine groups for affinity purification or assay detection of antibodies, antigens and other biomolecules. Compared with the traditional beads with carboxyl or amino groups, the beads with surface containing NHS group do not need to be activated by EDC / NHS or glutaraldehyde in advance. Simply by dissolving the ligand containing the primary amino acid in the coupling buffer (mix the protein solution with Mag NHS beads at room temperature for 1 ~ 2h), the ligands will be attached on the beads.
||Polymer NHS Magbeads Preactivated
|Mean particle size
||~50 μM/mL gel
||20~30 mg rabbit IgG/mL beads
|* water average particle size, Malvern Nano determination
1. Simple, without activation, can be directly covalently coupled with the biological ligand;
2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;
3. Fast, 1 ~ 2h to complete the bio-ligand coupling;
4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;
5. Stable, the formation of a stable amide bond, to prevent the ligand off.
6. Good biocompatibility, reduced nonspecific adsorption.
1. Magnetic beads are sensitive to water. In order to ensure product quality, after sampling immediately cover the bottle caps and seal with a sealing tape, store at 4 ℃.
2. Beads are not allowed to dry or freeze. Drying and freezing operations may result in the aggregation of magnetic beads and thus loss of binding activity.
3. Pre-and post-reaction protein content can be determined by indirect methods (e.g., Thermo Scientific™ Pierce™ 660 nm Protein Assay, Product No. 22660 and 22662) or by direct methods (e.g., use the Thermo Scientific™ Pierce™ Micro BCA Protein Assay, Product No. 23235) to detect the surface of the magnetic beads coupled with the protein content. Using wavelengths around 280 nm to determine protein levels is
not advisable. Because NHS groups strongly absorb near 280nm, they can seriously interfere with the detection.